Hotspot

clpP

LSC · 72087-74179 · 2093 bp

Back to Rhopaloblaste

Pi 0.000478

MarkerSeek score 57.80

Primer available yes

Label clpP

Feature Metadata

label_name: clpP
feature_type: gene
region: LSC
length_bp: 2093
variable_sites: 1
pi: 0.000478
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
clpP_p1	GGAATGGGTTGGTTTAGATCGA	AACCCATTGTTACGTTTCCA	45.5	40.0	58.1	55.5	2263 bp	1.0	42.8
clpP_p2	GGAATGGGTTGGTTTAGATCGA	AAACCCATTGTTACGTTTCCA	45.5	38.1	58.1	56.3	2264 bp	1.0	41.2
clpP_p3	GGAATGGGTTGGTTTAGATCG	AACCCATTGTTACGTTTCCA	47.6	40.0	56.7	55.5	2263 bp	1.0	41.1
clpP_p4	GGAATGGGTTGGTTTAGATCGA	ACCCATTGTTACGTTTCCAT	45.5	40.0	58.1	55.2	2262 bp	1.0	41.0
clpP_p5	TGGAATGGGTTGGTTTAGATCG	AACCCATTGTTACGTTTCCA	45.5	40.0	58.4	55.5	2264 bp	1.0	40.7