Hotspot

accD

LSC · 59254-60768 · 1515 bp

Back to Rhopaloblaste

Pi 0.00066

MarkerSeek score 59.10

Primer available yes

Label accD

Feature Metadata

label_name: accD
feature_type: gene
region: LSC
length_bp: 1515
variable_sites: 1
pi: 0.00066
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD_p1	ATAGGGGGCAAGAAGACTCT	AGAAAACACCATCCTTTTTCGT	50.0	36.4	57.4	56.7	1620 bp	1.0	49.3
accD_p2	ATAGGGGGCAAGAAGACTCT	TGTCATCAAAGAAAACACCATCC	50.0	39.1	57.4	57.5	1629 bp	1.0	47.8
accD_p3	ATAGGGGGCAAGAAGACTCT	AAGAAAACACCATCCTTTTTCGT	50.0	34.8	57.4	57.4	1621 bp	1.0	46.7
accD_p4	ATAGGGGGCAAGAAGACTCT	AGAAAACACCATCCTTTTTCGTT	50.0	34.8	57.4	57.4	1620 bp	1.0	46.7
accD_p5	ATAGGGGGCAAGAAGACTCT	GTCATCAAAGAAAACACCATCCT	50.0	39.1	57.4	57.3	1628 bp	1.0	45.7