Hotspot

psaC-ndhE

SSC · 120005-120469 · 465 bp

Back to Rhopaloblaste

Pi 0.0152

MarkerSeek score 64.70

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 465
variable_sites: 7
pi: 0.0152
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	ACATCCGTCCCAAGGTATCA	CTGAAGCAGCTATTGGGCTA	50.0	50.0	58.1	57.4	659-663 bp	1.0	74.9
psaC-ndhE_p2	ACATCCGTCCCAAGGTATCA	TGAAGCAGCTATTGGGCTAG	50.0	50.0	58.1	57.4	658-662 bp	1.0	74.9
psaC-ndhE_p3	ACATCCGTCCCAAGGTATCA	TGTTATAGCTGTTGCGGCTG	50.0	50.0	58.1	58.6	679-683 bp	1.0	74.9
psaC-ndhE_p4	ACATCCGTCCCAAGGTATCA	GAAGCAGCTATTGGGCTAGC	50.0	55.0	58.1	58.8	657-661 bp	1.0	73.8
psaC-ndhE_p5	CAATACATCTGTGGGGCAGG	TGAAGCAGCTATTGGGCTAG	55.0	50.0	58.3	57.4	634-638 bp	1.0	73.5