Hotspot

trnN-GUU-ndhF

IRb · 111911-113311 · 1401 bp

Back to Rhopaloblaste

Pi 0.000714

MarkerSeek score 59.10

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 1401
variable_sites: 1
pi: 0.000714
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	AGTCAGTTAACAGCCAACCG	GGGAGGGCGAATCTCATCTT	50.0	55.0	58.1	59.2	1559-1641 bp	1.0	69.2
trnN-GUU-ndhF_p2	TCTACCACTGAGCTACTGGG	GGGAGGGCGAATCTCATCTT	55.0	55.0	57.9	59.2	1538-1620 bp	1.0	69.0
trnN-GUU-ndhF_p3	ACGACCAGTCAGTTAACAGC	GGGAGGGCGAATCTCATCTT	50.0	55.0	57.8	59.2	1565-1647 bp	1.0	68.9
trnN-GUU-ndhF_p4	AGGATTCGAACCTACGACCA	GGGAGGGCGAATCTCATCTT	50.0	55.0	58.2	59.2	1578-1660 bp	1.0	68.9
trnN-GUU-ndhF_p5	GGATTCGAACCTACGACCAG	GGGAGGGCGAATCTCATCTT	55.0	55.0	57.8	59.2	1577-1659 bp	1.0	68.5