Hotspot

rpl20-rps12

LSC · 71125-71836 · 712 bp

Back to Rhopaloblaste

Pi 0

MarkerSeek score 40.00

Primer available yes

Label rpl20-rps12

Feature Metadata

label_name: rpl20-rps12
feature_type: igs
region: LSC
length_bp: 712
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20-rps12_p1	CGACGTTTCCGAGCTGTATA	TCTCCCGCTCTTAGAGGATG	50.0	55.0	57.5	58.0	807 bp	1.0	76.2
rpl20-rps12_p2	CGACGTTTCCGAGCTGTATA	GAGGAACATGCACTAGGGTG	50.0	55.0	57.5	58.0	776 bp	1.0	76.1
rpl20-rps12_p3	CGACGTTTCCGAGCTGTATA	GTCGAGGAACATGCACTAGG	50.0	55.0	57.5	58.1	779 bp	1.0	75.7
rpl20-rps12_p4	TCCGAGCTGTATATCCTCGT	TCTCCCGCTCTTAGAGGATG	50.0	55.0	57.4	58.0	800 bp	1.0	75.4
rpl20-rps12_p5	TCCGAGCTGTATATCCTCGT	GAGGAACATGCACTAGGGTG	50.0	55.0	57.4	58.0	769 bp	1.0	75.3