Hotspot

ndhC-trnV-UAC

LSC · 51373-53251 · 1879 bp

Back to Rhopaloblaste

Pi 0

MarkerSeek score 58.30

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1879
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TCCTTCACTAACCGGTGCTA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.1	57.8	2017-2023 bp	1.0	78.1
ndhC-trnV-UAC_p2	TAACCGGTGCTAAAACTCCG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.9	57.8	2009-2015 bp	1.0	78.0
ndhC-trnV-UAC_p3	TCCTTCACTAACCGGTGCTA	AGCCCAATGCAACAAAATGC	50.0	45.0	58.1	58.5	2099-2105 bp	1.0	75.9
ndhC-trnV-UAC_p4	TAACCGGTGCTAAAACTCCG	AGCCCAATGCAACAAAATGC	50.0	45.0	57.9	58.5	2091-2097 bp	1.0	75.8
ndhC-trnV-UAC_p5	TCCTTCACTAACCGGTGCTA	GAAGGTCTACGGTTCGAGTC	50.0	55.0	58.1	57.5	2016-2022 bp	1.0	75.3