Hotspot

trnL-UAA-trnF-GAA

LSC · 48514-48861 · 348 bp

Back to Rhopaloblaste

Pi 0

MarkerSeek score 52.10

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 348
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 52.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AATCGTGAGGGTTCAAGTCC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	438 bp	1.0	75.8
trnL-UAA-trnF-GAA_p2	AATCGTGAGGGTTCAAGTCC	TACCAACTGAGCTATCCCGA	50.0	50.0	57.5	57.6	299-400 bp	1.0	72.6
trnL-UAA-trnF-GAA_p3	AATCGTGAGGGTTCAAGTCC	GAACTGGTGACACGAGGATT	50.0	50.0	57.5	57.5	300-435 bp	1.0	72.5
trnL-UAA-trnF-GAA_p4	TTCAAGTCCCTCTATCCCCA	TTTGAACTGGTGACACGAGG	50.0	50.0	57.0	58.1	427 bp	1.0	71.9
trnL-UAA-trnF-GAA_p5	AATCGTGAGGGTTCAAGTCC	TCCTCTGCTCTACCAACTGA	50.0	50.0	57.5	57.4	410 bp	1.0	71.4