Hotspot

ycf1

SSC · 122147-127717 · 5571 bp

Back to Rhomboda

Pi 0.00682

MarkerSeek score 52.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5571
variable_sites: 38
pi: 0.00682
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TCATCTACCGAATATGTCACACA	CAAATTGTTCCTTTGGCCCA	39.1	45.0	57.2	57.1	202 bp	1.0	43.8
ycf1_p2	TCATCTACCGAATATGTCACACA	TCCTTTGGCCCAATTATCGA	39.1	45.0	57.2	56.9	194 bp	1.0	42.4
ycf1_p3	TCATCTACCGAATATGTCACACA	CAAATTGTTCCTTTGGCCCAA	39.1	42.9	57.2	57.8	202 bp	1.0	42.0
ycf1_p4	TCATCTACCGAATATGTCACACA	TCAAATTGTTCCTTTGGCCC	39.1	45.0	57.2	56.8	203 bp	1.0	42.0
ycf1_p5	AGCTTTCATCTACCGAATATGTCA	CAAATTGTTCCTTTGGCCCA	37.5	45.0	57.8	57.1	207 bp	1.0	41.9