Hotspot

rps15-ycf1

SSC · 121811-122146 · 336 bp

Back to Rhomboda

Pi 0.0119

MarkerSeek score 46.50

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 336
variable_sites: 4
pi: 0.0119
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 46.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	GATCAGCCGAAGAACACCAT	CGAAATGCTGATCCAAAGGG	50.0	50.0	58.0	57.1	1469-1477 bp	1.0	73.8
rps15-ycf1_p2	TTTCCAATCGTTCTGGTGCA	CGAAATGCTGATCCAAAGGG	45.0	50.0	58.0	57.1	1653-1661 bp	1.0	73.7
rps15-ycf1_p3	AACGAGTTTTTGGTTCCCGA	CGAAATGCTGATCCAAAGGG	45.0	50.0	58.0	57.1	1241-1249 bp	1.0	73.6
rps15-ycf1_p4	ACGAGTTTTTGGTTCCCGAA	CGAAATGCTGATCCAAAGGG	45.0	50.0	58.0	57.1	1240-1248 bp	1.0	73.6
rps15-ycf1_p5	AACACCATGCATTGATGGGT	CGAAATGCTGATCCAAAGGG	45.0	50.0	58.1	57.1	1457-1465 bp	1.0	73.5