Hotspot

petB-petD

LSC · 74907-75889 · 983 bp

Back to Rhomboda

Pi 0.00411

MarkerSeek score 62.00

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 983
variable_sites: 4
pi: 0.00411
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	AAGCAAGGTATTTCGGGTCC	GATCCATCCCCCAAAGGAAC	50.0	55.0	57.9	57.9	1028-1037 bp	1.0	79.4
petB-petD_p2	GCAAGGTATTTCGGGTCCTT	GATCCATCCCCCAAAGGAAC	50.0	55.0	57.9	57.9	1026-1035 bp	1.0	79.4
petB-petD_p3	AAGCAAGGTATTTCGGGTCC	ATGCCCCATCCCTTTAGCTA	50.0	50.0	57.9	58.2	1115-1124 bp	1.0	78.6
petB-petD_p4	GCAAGGTATTTCGGGTCCTT	ATGCCCCATCCCTTTAGCTA	50.0	50.0	57.9	58.2	1113-1122 bp	1.0	78.6
petB-petD_p5	GCAAGGTATTTCGGGTCCTT	TATGCCCCATCCCTTTAGCT	50.0	50.0	57.9	58.2	1114-1123 bp	1.0	78.6