Hotspot

psbZ-trnG-UCC

LSC · 35208-35481 · 274 bp

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Pi 0
MarkerSeek score 23.50
Primer available yes
Label psbZ-trnG-UCC

Feature Metadata

label_name
psbZ-trnG-UCC
feature_type
igs
region
LSC
length_bp
274
variable_sites
0
pi
0
unique_haplotype_count
1
primer_available
yes
alignment_reliability
1.00
markerseek_score
23.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-UCC_p1 AGTCTTTCTGGTAGCTATCCTGA AATCGAACCCGCATCTTCTC 43.5 50.0 58.1 58.1 372-418 bp 1.0 54.5
psbZ-trnG-UCC_p2 AGTCTTTCTGGTAGCTATCCTGA GAATCGAACCCGCATCTTCT 43.5 50.0 58.1 58.1 373-419 bp 1.0 54.5
psbZ-trnG-UCC_p3 AGTCTTTCTGGTAGCTATCCTGA TACTTCACCATGGGCGGATA 43.5 50.0 58.1 58.2 397-443 bp 1.0 53.4
psbZ-trnG-UCC_p4 GTCTTTCTGGTAGCTATCCTGA AATCGAACCCGCATCTTCTC 45.5 50.0 56.7 58.1 371-417 bp 1.0 53.3
psbZ-trnG-UCC_p5 GTCTTTCTGGTAGCTATCCTGA GAATCGAACCCGCATCTTCT 45.5 50.0 56.7 58.1 372-418 bp 1.0 53.3