Hotspot

rpoB-trnC-GCA

LSC · 26088-27316 · 1229 bp

Back to Rhomboda

Pi 0.00895

MarkerSeek score 63.90

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1229
variable_sites: 11
pi: 0.00895
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGTCAAGCCCCGATTAATGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.1	57.5	1369-1376 bp	1.0	76.0
rpoB-trnC-GCA_p2	CGTCAAGCCCCGATTAATGA	CGACACCCAGATTCGAACTG	50.0	55.0	58.1	58.6	1393-1400 bp	1.0	75.0
rpoB-trnC-GCA_p3	TCAATTCTTCCGTCAAGCCC	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.2	57.5	1379-1386 bp	1.0	75.0
rpoB-trnC-GCA_p4	CGTCAAGCCCCGATTAATGA	TTTGTTGATCAGGCGACACC	50.0	50.0	58.1	58.8	1406-1413 bp	1.0	74.1
rpoB-trnC-GCA_p5	TCAATTCTTCCGTCAAGCCC	CGACACCCAGATTCGAACTG	50.0	55.0	58.2	58.6	1403-1410 bp	1.0	74.0