Hotspot

psbK-psbI

LSC · 7106-7573 · 468 bp

Back to Rhomboda

Pi 0.0257

MarkerSeek score 71.00

Primer available yes

Label psbK-psbI

Feature Metadata

label_name: psbK-psbI
feature_type: igs
region: LSC
length_bp: 468
variable_sites: 12
pi: 0.0257
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbK-psbI_p1	GTTTGGCAAGCTGCTGTAAG	GGATTACGTCCCGGATCATT	50.0	50.0	57.9	57.2	592-593 bp	1.0	74.6
psbK-psbI_p2	GTTTGGCAAGCTGCTGTAAG	TCTTCACGTCCAGGATTACG	50.0	50.0	57.9	57.1	604-605 bp	1.0	73.8
psbK-psbI_p3	TGGCAAGCTGCTGTAAGTTT	GGATTACGTCCCGGATCATT	45.0	50.0	58.3	57.2	589-590 bp	1.0	72.6
psbK-psbI_p4	TTGGCAAGCTGCTGTAAGTT	GGATTACGTCCCGGATCATT	45.0	50.0	58.3	57.2	590-591 bp	1.0	72.6
psbK-psbI_p5	TTTGGCAAGCTGCTGTAAGT	GGATTACGTCCCGGATCATT	45.0	50.0	58.3	57.2	591-592 bp	1.0	72.6