Hotspot

trnQ-UUG-psbK

LSC · 6554-6919 · 366 bp

Back to Rhomboda

Pi 0.0191

MarkerSeek score 63.90

Primer available yes

Label trnQ-UUG-psbK

Feature Metadata

label_name: trnQ-UUG-psbK
feature_type: igs
region: LSC
length_bp: 366
variable_sites: 7
pi: 0.0191
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnQ-UUG-psbK_p1	GAAGGATTCGAACCTCCGAG	GGGCAATTTGGCGAAGAAAA	55.0	45.0	57.8	57.8	511 bp	1.0	78.1
trnQ-UUG-psbK_p2	AGGATTCGAACCTCCGAGTA	GGGCAATTTGGCGAAGAAAA	50.0	45.0	57.6	57.8	509 bp	1.0	76.3
trnQ-UUG-psbK_p3	AAGGATTCGAACCTCCGAGT	GGGCAATTTGGCGAAGAAAA	50.0	45.0	58.4	57.8	510 bp	1.0	76.3
trnQ-UUG-psbK_p4	GAGTAACGGGACCAAAACCC	GGGCAATTTGGCGAAGAAAA	55.0	45.0	58.5	57.8	494 bp	1.0	76.1
trnQ-UUG-psbK_p5	ATTCGAACCTCCGAGTAACG	GGGCAATTTGGCGAAGAAAA	50.0	45.0	57.4	57.8	506 bp	1.0	75.4