Hotspot

trnK-UUU-rps16

LSC · 4323-5020 · 698 bp

Back to Rhomboda

Pi 0.013
MarkerSeek score 53.50
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
698
variable_sites
9
pi
0.013
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 TTTAAGGATCAGTCGCGGTC GCAAAGACAACGAATTCCGG 50.0 50.0 57.7 58.0 1405-1407 bp 1.0 78.7
trnK-UUU-rps16_p2 AAAGCCGAGTACTCTACCGT GCAAAGACAACGAATTCCGG 50.0 50.0 58.2 58.0 1316 bp 0.5 58.4
trnK-UUU-rps16_p3 AAGCCGAGTACTCTACCGTT GCAAAGACAACGAATTCCGG 50.0 50.0 58.2 58.0 1315 bp 0.5 58.4
trnK-UUU-rps16_p4 AAAGCCGAGTACTCTACCGT TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.2 57.9 907 bp 0.5 57.5
trnK-UUU-rps16_p5 AAGCCGAGTACTCTACCGTT TCGATCCCGAAGAGAAGGAA 50.0 50.0 58.2 57.9 906 bp 0.5 57.5