Hotspot

ccsA-ndhD

SSC · 119608-119910 · 303 bp

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Pi 0.0221

MarkerSeek score 61.00

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 303
variable_sites: 10
pi: 0.0221
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CAAATTCCGCAATTGTGGCT	CGGTTGACAAGGTCGAAACT	45.0	50.0	57.9	58.4	430-469 bp	1.0	81.7
ccsA-ndhD_p2	CAAATTCCGCAATTGTGGCT	TCGGTTGACAAGGTCGAAAC	45.0	50.0	57.9	58.4	431-470 bp	1.0	81.7
ccsA-ndhD_p3	TGTGGCTTCTATGGGCTTTC	CGGTTGACAAGGTCGAAACT	50.0	50.0	57.9	58.4	417-456 bp	1.0	81.4
ccsA-ndhD_p4	TGTGGCTTCTATGGGCTTTC	TCGGTTGACAAGGTCGAAAC	50.0	50.0	57.9	58.4	418-457 bp	1.0	81.4
ccsA-ndhD_p5	TTCCGCAATTGTGGCTTCTA	CGGTTGACAAGGTCGAAACT	45.0	50.0	57.8	58.4	426-465 bp	1.0	80.9