Hotspot

rpl32-trnL-UAG

SSC · 117810-118468 · 659 bp

Back to Rhodoleia

Pi 0.00306

MarkerSeek score 61.50

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 659
variable_sites: 3
pi: 0.00306
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	GGGATATTGGGCAGCGTTAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	58.0	58.0	784-785 bp	1.0	80.7
rpl32-trnL-UAG_p2	GGGATATTGGGCAGCGTTAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	58.0	58.0	785-786 bp	1.0	80.6
rpl32-trnL-UAG_p3	GGGATATTGGGCAGCGTTAA	TTTAGAAGATCCCATGCCGC	50.0	50.0	58.0	58.0	849-850 bp	1.0	80.4
rpl32-trnL-UAG_p4	GGGATATTGGGCAGCGTTAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	58.0	57.9	794-795 bp	1.0	79.7
rpl32-trnL-UAG_p5	GGGCAGCGTTAAAAGCTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.9	58.0	776-777 bp	1.0	79.7