Hotspot

ndhF-rpl32

SSC · 116783-117644 · 862 bp

Back to Rhodoleia

Pi 0.00309

MarkerSeek score 60.70

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 862
variable_sites: 4
pi: 0.00309
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GCCGTCGGAAAAAGGAAAAG	TTAACGCTGCCCAATATCCC	50.0	50.0	58.0	58.0	1045-1048 bp	1.0	79.7
ndhF-rpl32_p2	GCCGTCGGAAAAAGGAAAAG	AAAAGCTTTTAACGCTGCCC	50.0	45.0	58.0	57.9	1053-1056 bp	1.0	78.7
ndhF-rpl32_p3	TTTGTTGCCGTCGGAAAAAG	TTAACGCTGCCCAATATCCC	45.0	50.0	57.8	58.0	1051-1054 bp	1.0	78.5
ndhF-rpl32_p4	CTGGAAGTGGAACGAAAGGT	TTAACGCTGCCCAATATCCC	50.0	50.0	57.7	58.0	1002-1005 bp	1.0	78.1
ndhF-rpl32_p5	TTTGTTGCCGTCGGAAAAAG	AAAAGCTTTTAACGCTGCCC	45.0	45.0	57.8	57.9	1059-1062 bp	1.0	77.5