Hotspot

ndhC-trnV-UAC

LSC · 53809-55000 · 1192 bp

Back to Rhodoleia

Pi 0.00677

MarkerSeek score 64.80

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1192
variable_sites: 12
pi: 0.00677
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	TGGTCCTTTACTAATCGGAGC	AGAAGGTCTACGGTTCGAGT	47.6	50.0	57.2	57.8	1328-1339 bp	1.0	65.8
ndhC-trnV-UAC_p2	TGGTCCTTTACTAATCGGAGC	AACGCATGTTGGGTCTTTGA	47.6	45.0	57.2	58.3	1395-1406 bp	1.0	64.9
ndhC-trnV-UAC_p3	TGGTCCTTTACTAATCGGAGC	ACGCATGTTGGGTCTTTGAA	47.6	45.0	57.2	58.3	1394-1405 bp	1.0	64.9
ndhC-trnV-UAC_p4	ACTCCGGAAATTAGAAATGCCA	AGAAGGTCTACGGTTCGAGT	40.9	50.0	58.0	57.8	1303-1314 bp	1.0	64.1
ndhC-trnV-UAC_p5	GGTCCTTTACTAATCGGAGCT	AGAAGGTCTACGGTTCGAGT	47.6	50.0	56.9	57.8	1327-1338 bp	1.0	63.5