Hotspot

rps4-trnT-UGU

LSC · 48831-49174 · 344 bp

Back to Rhodoleia

Pi 0.00969

MarkerSeek score 51.30

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 344
variable_sites: 5
pi: 0.00969
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	AGATCACTTCCGGCTCTAGG	CTCAGAGGTTAGAGCATCGC	55.0	55.0	58.3	57.9	502-539 bp	1.0	77.3
rps4-trnT-UGU_p2	AGATCACTTCCGGCTCTAGG	GGTTCGATTCCGATAGCTGG	55.0	55.0	58.3	58.2	461-498 bp	1.0	76.7
rps4-trnT-UGU_p3	AGATCACTTCCGGCTCTAGG	ATGGTCATCGGTTCGATTCC	55.0	50.0	58.3	57.8	470-507 bp	1.0	76.5
rps4-trnT-UGU_p4	AGATCACTTCCGGCTCTAGG	CTGTTATGTGAGCCCGCTTA	55.0	50.0	58.3	57.7	523-560 bp	1.0	75.9
rps4-trnT-UGU_p5	AGATCACTTCCGGCTCTAGG	TGTTATGTGAGCCCGCTTAG	55.0	50.0	58.3	57.7	522-559 bp	1.0	75.9