Hotspot

atpF-atpH

LSC · 14183-14574 · 392 bp

Back to Rhodoleia

Pi 0.00689

MarkerSeek score 61.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 392
variable_sites: 4
pi: 0.00689
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	572-576 bp	1.0	75.5
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	CGAGGCGGAGGGAAAAATAC	50.0	55.0	57.9	58.7	577-581 bp	1.0	75.4
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	GAGGCGGAGGGAAAAATACG	50.0	55.0	57.9	58.7	576-580 bp	1.0	75.4
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	GCGGAGGGAAAAATACGAGG	50.0	55.0	57.9	58.7	573-577 bp	1.0	75.4
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	GGCGGAGGGAAAAATACGAG	50.0	55.0	57.9	58.7	574-578 bp	1.0	75.4