Hotspot

rps16-trnQ-UUG

LSC · 6424-8170 · 1747 bp

Back to Rhodoleia

Pi 0.00422
MarkerSeek score 58.20
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1747
variable_sites
11
pi
0.00422
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.99
markerseek_score
58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1820-1832 bp 1.0 79.2
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1831-1843 bp 1.0 78.6
rps16-trnQ-UUG_p3 CCACAGCTGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 1841-1853 bp 1.0 76.9
rps16-trnQ-UUG_p4 TCCACAGCTGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 1842-1854 bp 1.0 76.9
rps16-trnQ-UUG_p5 ATCCACAGCTGATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.4 58.1 1843-1855 bp 1.0 75.2