Hotspot

rps19-trnI-CAU

IRa · 176969-179878 · 2910 bp

Back to Rhododendron

Pi 0.0978
MarkerSeek score 83.90
Primer available yes
Label rps19-trnI-CAU

Feature Metadata

label_name
rps19-trnI-CAU
feature_type
igs
region
IRa
length_bp
2910
variable_sites
1022
pi
0.0978
unique_haplotype_count
22
primer_available
yes
alignment_reliability
0.96
markerseek_score
83.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-trnI-CAU_p1 CGTGTAGGTGCACTATTCCG GTTGGGCGCTTTAACCATTC 55.0 50.0 58.4 58.0 925-2985 bp 0.772727 79.3
rps19-trnI-CAU_p2 GCGGTGGGGATTGTAACTTT GTTGGGCGCTTTAACCATTC 50.0 50.0 58.5 58.0 906-2966 bp 0.772727 79.2
rps19-trnI-CAU_p3 GCGTGTAGGTGCACTATTCC GTTGGGCGCTTTAACCATTC 55.0 50.0 58.7 58.0 926-2986 bp 0.772727 77.1
rps19-trnI-CAU_p4 GGCGTGTAGGTGCACTATTC GTTGGGCGCTTTAACCATTC 55.0 50.0 58.7 58.0 927-2987 bp 0.772727 77.1
rps19-trnI-CAU_p5 GTGTAGGTGCACTATTCCGC GTTGGGCGCTTTAACCATTC 55.0 50.0 58.7 58.0 924-2984 bp 0.772727 77.1