Hotspot

psaI-rps19

IRa · 170856-172839 · 1984 bp

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Pi 0.0235

MarkerSeek score 76.30

Primer available yes

Label psaI-rps19

Feature Metadata

label_name: psaI-rps19
feature_type: igs
region: IRa
length_bp: 1984
variable_sites: 260
pi: 0.0235
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-rps19_p1	GCCGGAAATACTAGGCCTAC	TGGTAGGTCACAAATTGGGAG	55.0	47.6	57.5	57.6	1511-2129 bp	0.909091	73.3
psaI-rps19_p2	GCCGGAAATACTAGGCCTAC	GCACCTACTTCGAATTTCCG	55.0	50.0	57.5	56.9	1485-2103 bp	0.818182	72.4
psaI-rps19_p3	GCCGGAAATACTAGGCCTAC	TGGGAGAATTTGCACCTACT	55.0	45.0	57.5	56.4	1496-2114 bp	0.909091	72.2
psaI-rps19_p4	GCCGGAAATACTAGGCCTAC	TGGTAGGTCACAAATTGGGA	55.0	45.0	57.5	56.3	1511-2129 bp	0.909091	71.5
psaI-rps19_p5	GCCGGAAATACTAGGCCTAC	GCACCTACTTCGAATTTCCGA	55.0	47.6	57.5	58.4	1485-2103 bp	0.818182	70.0