Hotspot

rps16-psaI

IRb · 143166-146989 · 3824 bp

Back to Rhododendron

Pi 0.0233

MarkerSeek score 69.90

Primer available yes

Label rps16-psaI

Feature Metadata

label_name: rps16-psaI
feature_type: igs
region: IRb
length_bp: 3824
variable_sites: 454
pi: 0.0233
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-psaI_p1	AACGAATCTGAGGGCAATCC	CCGGTACAGTTGTGACGATT	50.0	50.0	58.0	57.9	440-459 bp	0.772727	80.1
rps16-psaI_p2	AACGAATCTGAGGGCAATCC	TTTTCCCAGATCGCGACAAT	50.0	45.0	58.0	57.9	560-579 bp	0.772727	78.7
rps16-psaI_p3	TCCCGGGCATCTACCATTAT	CCGGTACAGTTGTGACGATT	50.0	50.0	58.0	57.9	791-825 bp	0.727273	78.2
rps16-psaI_p4	TCCCGGGCATCTACCATTAT	TTTTCCCAGATCGCGACAAT	50.0	45.0	58.0	57.9	911-945 bp	0.727273	77.4
rps16-psaI_p5	TCCCGGGCATCTACCATTAT	AATTGCGGTTCCACCTAGAC	50.0	50.0	58.0	57.9	958-992 bp	0.727273	77.3