Hotspot

trnI-CAU-trnM-CAU

IRb · 135151-137605 · 2455 bp

Back to Rhododendron

Pi 0.103

MarkerSeek score 75.50

Primer available yes

Label trnI-CAU-trnM-CAU

Feature Metadata

label_name: trnI-CAU-trnM-CAU
feature_type: igs
region: IRb
length_bp: 2455
variable_sites: 1012
pi: 0.103
unique_haplotype_count: 20
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 75.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-trnM-CAU_p1	CGCTTAACCATTCAGCCATG	CGATAGGTCCCGTTTGGTTT	50.0	50.0	57.5	57.9	2596-2603 bp	0.090909	40.2
trnI-CAU-trnM-CAU_p2	CGCTTAACCATTCAGCCATG	TACGATAGGTCCCGTTTGGT	50.0	50.0	57.5	58.1	2598-2605 bp	0.090909	39.8
trnI-CAU-trnM-CAU_p3	CGCTTAACCATTCAGCCATG	ATAGGTCCCGTTTGGTTTGG	50.0	50.0	57.5	57.8	2594-2601 bp	0.090909	39.3
trnI-CAU-trnM-CAU_p4	CGCTTAACCATTCAGCCATG	CCCGTTTGGTTTGGTTCTCT	50.0	50.0	57.5	58.3	2588-2595 bp	0.090909	38.5
trnI-CAU-trnM-CAU_p5	CGCTTAACCATTCAGCCATG	TCCCGTTTGGTTTGGTTCTC	50.0	50.0	57.5	58.3	2589-2596 bp	0.090909	38.5