Hotspot

trnI-CAU-rrn16S

IRb · 111883-114339 · 2457 bp

Back to Rhododendron

Pi 0.0832

MarkerSeek score 82.80

Primer available yes

Label trnI-CAU-rrn16S

Feature Metadata

label_name: trnI-CAU-rrn16S
feature_type: igs
region: IRb
length_bp: 2457
variable_sites: 740
pi: 0.0832
unique_haplotype_count: 21
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-rrn16S_p1	CCCATTAGCATGCATCCAGT	GGATCGAACTCTCCGTGAGA	50.0	55.0	58.0	58.6	2309-2942 bp	0.727273	76.1
trnI-CAU-rrn16S_p2	GTTGGGCGCTTTAACCATTC	GGATCGAACTCTCCGTGAGA	50.0	55.0	58.0	58.6	2257-2890 bp	0.727273	76.1
trnI-CAU-rrn16S_p3	GTCCCATTAGCATGCATCCA	GGATCGAACTCTCCGTGAGA	50.0	55.0	58.0	58.6	2311-2944 bp	0.727273	76.0
trnI-CAU-rrn16S_p4	CCCATTAGCATGCATCCAGT	CTGAGCCAGGATCGAACTCT	50.0	55.0	58.0	58.9	2317-2950 bp	0.727273	73.9
trnI-CAU-rrn16S_p5	GTTGGGCGCTTTAACCATTC	CTGAGCCAGGATCGAACTCT	50.0	55.0	58.0	58.9	2265-2898 bp	0.727273	73.9