Hotspot

trnE-UUC-psbB

LSC · 87285-89071 · 1787 bp

Back to Rhododendron

Pi 0.0169

MarkerSeek score 71.50

Primer available yes

Label trnE-UUC-psbB

Feature Metadata

label_name: trnE-UUC-psbB
feature_type: igs
region: LSC
length_bp: 1787
variable_sites: 139
pi: 0.0169
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-psbB_p1	TTTCGTAGTACCCTACCCCC	CGATACCAAGGCAAACCCAT	55.0	50.0	57.8	58.2	1887-1921 bp	1.0	84.0
trnE-UUC-psbB_p2	GCTGCCTCCTTGAAAGAGAG	CGATACCAAGGCAAACCCAT	55.0	50.0	58.3	58.2	1849-1883 bp	1.0	83.3
trnE-UUC-psbB_p3	TGTCCTGAACCACTAGACGA	CGATACCAAGGCAAACCCAT	50.0	50.0	57.7	58.2	1828-1862 bp	1.0	83.2
trnE-UUC-psbB_p4	TTTCGTAGTACCCTACCCCC	ATACAGAGCCATTGAACCGG	55.0	50.0	57.8	57.7	1987-2021 bp	1.0	82.9
trnE-UUC-psbB_p5	TTTCGTAGTACCCTACCCCC	AGCAACCAAAGCTGTATGCA	55.0	45.0	57.8	58.4	1960-1994 bp	1.0	82.6