Hotspot

rpoA-rps15

LSC · 65608-72989 · 7382 bp

Back to Rhododendron

Pi 0.0552

MarkerSeek score 69.70

Primer available yes

Label rpoA-rps15

Feature Metadata

label_name: rpoA-rps15
feature_type: igs
region: LSC
length_bp: 7382
variable_sites: 1385
pi: 0.0552
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.65
markerseek_score: 69.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoA-rps15_p1	GTTGAGGGAAAGGACGATGG	TGGCGTTGGATCAGAATAGC	55.0	50.0	58.3	58.0	1505 bp	0.045455	39.4
rpoA-rps15_p2	CGATGGCCCCAAATACTCTT	TGGCGTTGGATCAGAATAGC	50.0	50.0	57.7	58.0	1491 bp	0.045455	38.7
rpoA-rps15_p3	GTTGAGGGAAAGGACGATGG	CTGCAATCTCAGGGTTTGTT	55.0	45.0	58.3	56.2	1485 bp	0.045455	25.5
rpoA-rps15_p4	CGATGGCCCCAAATACTCTT	CTGCAATCTCAGGGTTTGTT	50.0	45.0	57.7	56.2	1471 bp	0.045455	24.8
rpoA-rps15_p5	GACGATGGCCCCAAATACTC	CTGCAATCTCAGGGTTTGTT	55.0	45.0	58.4	56.2	1473 bp	0.045455	24.4