Hotspot

trnT-GGU-rpl16

LSC · 54408-60945 · 6538 bp

Back to Rhododendron

Pi 0.0611

MarkerSeek score 73.30

Primer available yes

Label trnT-GGU-rpl16

Feature Metadata

label_name: trnT-GGU-rpl16
feature_type: igs
region: LSC
length_bp: 6538
variable_sites: 1376
pi: 0.0611
unique_haplotype_count: 22
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 73.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-rpl16_p1	TATTCACGCAGGGAGCATTC	GCGATTTTCTGAACCAGCAC	50.0	50.0	58.0	58.0	684-696 bp	0.590909	75.0
trnT-GGU-rpl16_p2	TCAGCTTGATGCAAATGGGT	GCGATTTTCTGAACCAGCAC	45.0	50.0	58.1	58.0	924-936 bp	0.590909	74.8
trnT-GGU-rpl16_p3	AACGAATCTGAGGGCAATCC	GCGATTTTCTGAACCAGCAC	50.0	50.0	58.0	58.0	374-392 bp	0.545455	73.2
trnT-GGU-rpl16_p4	TGCCGGCATCTACCACTATA	GCGATTTTCTGAACCAGCAC	50.0	50.0	58.0	58.0	463 bp	0.090909	43.4
trnT-GGU-rpl16_p5	TGCCGGCATCTACCACTATA	CGAAGGACTGTGACATAGCC	50.0	55.0	58.0	58.1	554 bp	0.090909	43.0