Hotspot

rpoC1

Genome · 73853-76620 · 2768 bp

Back to Rhodobryum

Pi 0.0372

MarkerSeek score 64.60

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: Genome
length_bp: 2768
variable_sites: 103
pi: 0.0372
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	AGAAGAGCATTTTGGCTGGT	AGAATCAGACCATTCCGTCA	45.0	45.0	57.7	56.3	2980 bp	0.5	43.6
rpoC1_p2	AGAGCATTTTGGCTGGTTCT	AGAATCAGACCATTCCGTCA	45.0	45.0	57.7	56.3	2977 bp	0.5	43.6
rpoC1_p3	GAGCATTTTGGCTGGTTCTG	AGAATCAGACCATTCCGTCA	50.0	45.0	57.6	56.3	2976 bp	0.5	43.2
rpoC1_p4	AGAAGAGCATTTTGGCTGGT	AGAATCAGACCATTCCGTCAT	45.0	42.9	57.7	56.8	2980 bp	0.5	40.0
rpoC1_p5	AGAGCATTTTGGCTGGTTCT	AGAATCAGACCATTCCGTCAT	45.0	42.9	57.7	56.8	2977 bp	0.5	40.0