Hotspot

trnC-GCA-ycf66

Genome · 80200-80683 · 484 bp

Back to Rhodobryum

Pi 0.0707

MarkerSeek score 74.60

Primer available yes

Label trnC-GCA-ycf66

Feature Metadata

label_name: trnC-GCA-ycf66
feature_type: igs
region: Genome
length_bp: 484
variable_sites: 34
pi: 0.0707
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-ycf66_p1	AACTACAAAGGTGCCTTGGG	TGGCGTTTGGATCCAATTCT	50.0	45.0	58.0	57.8	1421 bp	0.5	58.2
trnC-GCA-ycf66_p2	ATCATGACTCTTGCGACACC	TGGCGTTTGGATCCAATTCT	50.0	45.0	58.0	57.8	1444 bp	0.5	58.2
trnC-GCA-ycf66_p3	AAGTGGTAAGGCAGAGGACT	TGGCGTTTGGATCCAATTCT	50.0	45.0	58.0	57.8	801 bp	0.5	58.0
trnC-GCA-ycf66_p4	GCGATATGGCCAAGTGGTAA	TGGCGTTTGGATCCAATTCT	50.0	45.0	58.0	57.8	812 bp	0.5	58.0
trnC-GCA-ycf66_p5	ATCCTTTACCCCCAGTTCGA	TGGCGTTTGGATCCAATTCT	50.0	45.0	58.0	57.8	777 bp	0.5	58.0