Hotspot

trnR-UCU-atpA

Genome · 64091-64238 · 148 bp

Back to Rhodobryum

Pi 0.136

MarkerSeek score 81.20

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: Genome
length_bp: 148
variable_sites: 20
pi: 0.136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGACAGGACAGAGGTCTTCT	GCAGAAATTGTACGTTCTACCA	55.0	40.9	58.1	56.7	311-312 bp	1.0	58.7
trnR-UCU-atpA_p2	GGACAGGACAGAGGTCTTCT	TGCAGAAATTGTACGTTCTACC	55.0	40.9	58.1	56.7	312-313 bp	1.0	58.7
trnR-UCU-atpA_p3	GGACAGGACAGAGGTCTTCT	TGCAGAAATTGTACGTTCTACCA	55.0	39.1	58.1	58.4	312-313 bp	1.0	57.9
trnR-UCU-atpA_p4	ATGGACAGGACAGAGGTCTT	GCAGAAATTGTACGTTCTACCA	50.0	40.9	57.7	56.7	313-314 bp	1.0	56.6
trnR-UCU-atpA_p5	ATGGACAGGACAGAGGTCTT	TGCAGAAATTGTACGTTCTACC	50.0	40.9	57.7	56.7	314-315 bp	1.0	56.6