Hotspot

trnS-GCU-psaM

Genome · 62349-62628 · 280 bp

Back to Rhodobryum

Pi 0.0566

MarkerSeek score 75.00

Primer available yes

Label trnS-GCU-psaM

Feature Metadata

label_name: trnS-GCU-psaM
feature_type: igs
region: Genome
length_bp: 280
variable_sites: 15
pi: 0.0566
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GCU-psaM_p1	TTAAACCACTCGGCCATCTC	TGTAGCTCTTGTCAGTGCTT	50.0	45.0	57.6	56.8	361-373 bp	1.0	73.7
trnS-GCU-psaM_p2	TAAACCACTCGGCCATCTCT	TGTAGCTCTTGTCAGTGCTT	50.0	45.0	58.4	56.8	360-372 bp	1.0	73.4
trnS-GCU-psaM_p3	TTAAACCACTCGGCCATCTC	TGTAGCTCTTGTCAGTGCTTT	50.0	42.9	57.6	57.5	361-373 bp	1.0	71.6
trnS-GCU-psaM_p4	TTAAACCACTCGGCCATCTC	TTGTAGCTCTTGTCAGTGCTT	50.0	42.9	57.6	57.5	362-374 bp	1.0	71.5
trnS-GCU-psaM_p5	TAAACCACTCGGCCATCTCT	TGTAGCTCTTGTCAGTGCTTT	50.0	42.9	58.4	57.5	360-372 bp	1.0	71.3