Hotspot

psbI-trnS-GCU

Genome · 62150-62262 · 113 bp

Back to Rhodobryum

Pi 0.0826

MarkerSeek score 75.90

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: Genome
length_bp: 113
variable_sites: 9
pi: 0.0826
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	AATGATCCTGGCCGTAATCC	GGGTTCGAATCCCTCTCTCT	50.0	55.0	57.4	58.2	168-171 bp	1.0	79.9
psbI-trnS-GCU_p2	TCAAATGATCCTGGCCGTAA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	56.9	58.2	171-174 bp	1.0	75.6
psbI-trnS-GCU_p3	AAATGATCCTGGCCGTAATCC	GGGTTCGAATCCCTCTCTCT	47.6	55.0	58.1	58.2	169-172 bp	1.0	75.3
psbI-trnS-GCU_p4	CTATCAAATGATCCTGGCCGT	GGGTTCGAATCCCTCTCTCT	47.6	55.0	57.9	58.2	174-177 bp	1.0	75.1
psbI-trnS-GCU_p5	ATCAAATGATCCTGGCCGTA	GGGTTCGAATCCCTCTCTCT	45.0	55.0	56.7	58.2	172-175 bp	1.0	73.7