Hotspot

ycf2-trnH-GUG

Genome · 54384-54756 · 373 bp

Back to Rhodobryum

Pi 0.0587

MarkerSeek score 76.20

Primer available yes

Label ycf2-trnH-GUG

Feature Metadata

label_name: ycf2-trnH-GUG
feature_type: igs
region: Genome
length_bp: 373
variable_sites: 21
pi: 0.0587
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 76.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-trnH-GUG_p1	TTTTGGAGAGAGACTGACGC	GCCCGTAGTAGGTATCTGGT	50.0	55.0	57.6	58.0	1233-1240 bp	1.0	80.5
ycf2-trnH-GUG_p2	TTTTGGAGAGAGACTGACGC	TGGCCCGTAGTAGGTATCTG	50.0	55.0	57.6	58.0	1235-1242 bp	1.0	80.5
ycf2-trnH-GUG_p3	TTTTGGAGAGAGACTGACGC	TGTTGATAGTCAAGGCCGTG	50.0	50.0	57.6	57.9	1149-1156 bp	1.0	79.9
ycf2-trnH-GUG_p4	TTTTGGAGAGAGACTGACGC	GTTGATAGTCAAGGCCGTGT	50.0	50.0	57.6	57.9	1148-1155 bp	1.0	79.9
ycf2-trnH-GUG_p5	TTTTGGAGAGAGACTGACGC	GGTGTAGCTGGTGTATTCGG	50.0	55.0	57.6	58.1	1466-1473 bp	1.0	79.7