Hotspot

trnF-GAA-trnL-UAA

Genome · 32939-32998 · 60 bp

Back to Rhodobryum

Pi 0.0667

MarkerSeek score 75.00

Primer available yes

Label trnF-GAA-trnL-UAA

Feature Metadata

label_name: trnF-GAA-trnL-UAA
feature_type: igs
region: Genome
length_bp: 60
variable_sites: 4
pi: 0.0667
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-trnL-UAA_p1	TCCTCTGCTCTACCAACTGA	CGTTGGCTTTATTGACCGTG	50.0	50.0	57.4	57.8	135 bp	1.0	75.6
trnF-GAA-trnL-UAA_p2	TCAGTCCTCTGCTCTACCAA	CGTTGGCTTTATTGACCGTG	50.0	50.0	57.4	57.8	139 bp	1.0	75.5
trnF-GAA-trnL-UAA_p3	TTCAGTCCTCTGCTCTACCA	CGTTGGCTTTATTGACCGTG	50.0	50.0	57.4	57.8	140 bp	1.0	75.5
trnF-GAA-trnL-UAA_p4	AGTCCTCTGCTCTACCAACT	CGTTGGCTTTATTGACCGTG	50.0	50.0	57.4	57.8	137 bp	1.0	75.5
trnF-GAA-trnL-UAA_p5	GTCCTCTGCTCTACCAACTG	CGTTGGCTTTATTGACCGTG	55.0	50.0	57.3	57.8	136 bp	1.0	75.1