Hotspot

rps12-rpl20

Genome · 14724-15659 · 936 bp

Back to Rhodobryum

Pi 0.046

MarkerSeek score 74.80

Primer available yes

Label rps12-rpl20

Feature Metadata

label_name: rps12-rpl20
feature_type: igs
region: Genome
length_bp: 936
variable_sites: 43
pi: 0.046
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rpl20_p1	CGGATGTCCTCAACGAAGAG	TGAGCTCCCTGAAATCCAGA	55.0	50.0	58.1	58.0	1059-1108 bp	1.0	82.2
rps12-rpl20_p2	ACAAAATCTCCAGCCCTTCG	TGAGCTCCCTGAAATCCAGA	50.0	50.0	58.2	58.0	1079-1128 bp	1.0	81.5
rps12-rpl20_p3	CTCAACGAAGAGGGGTTTGT	TGAGCTCCCTGAAATCCAGA	50.0	50.0	57.7	58.0	1051-1100 bp	1.0	80.9
rps12-rpl20_p4	CGGATGTCCTCAACGAAGAG	GCTCCCTGAAATCCAGATGT	55.0	50.0	58.1	57.3	1056-1105 bp	1.0	76.8
rps12-rpl20_p5	ACAAAATCTCCAGCCCTTCG	GCTCCCTGAAATCCAGATGT	50.0	50.0	58.2	57.3	1076-1125 bp	1.0	76.0