Hotspot

trnI-CAU-rpl23

Genome · 145-386 · 242 bp

Back to Rhodobryum

Pi 0.0996

MarkerSeek score 73.40

Primer available yes

Label trnI-CAU-rpl23

Feature Metadata

label_name: trnI-CAU-rpl23
feature_type: igs
region: Genome
length_bp: 242
variable_sites: 24
pi: 0.0996
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 73.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnI-CAU-rpl23_p1	TGAACGGTTAAAGCACCCAA	AAACGTTTGTGACCTCCTCC	45.0	50.0	57.9	58.0	791-797 bp	1.0	83.8
trnI-CAU-rpl23_p2	AACGGTTAAAGCACCCAACT	AAACGTTTGTGACCTCCTCC	45.0	50.0	57.9	58.0	789-795 bp	1.0	83.8
trnI-CAU-rpl23_p3	CCCAACTCATAATTGGCGGA	AAACGTTTGTGACCTCCTCC	50.0	50.0	57.9	58.0	776 bp	0.5	59.3
trnI-CAU-rpl23_p4	GATTCGCAGGTTCAACTCCT	AAACGTTTGTGACCTCCTCC	50.0	50.0	57.9	58.0	758 bp	0.5	58.9
trnI-CAU-rpl23_p5	CCCAACTCATAATTGGCGGA	TTTGTGACCTCCTCCTCTGT	50.0	50.0	57.9	57.9	771 bp	0.5	58.8