Hotspot

ndhF

SSC · 107961-110177 · 2217 bp

Back to Rhodiola

Pi 0.011

MarkerSeek score 58.20

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2217
variable_sites: 176
pi: 0.011
unique_haplotype_count: 46
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	GGAAGAGATCCGAGTGAACG	TGGCCAGAGAAGTACGTTTT	55.0	45.0	57.8	57.1	2956-3000 bp	0.86	74.9
ndhF_p2	GGAAGAGATCCGAGTGAACG	TTGGCCAGAGAAGTACGTTT	55.0	45.0	57.8	57.1	2957-2997 bp	0.82	72.8
ndhF_p3	GGAAGAGATCCGAGTGAACG	TTTGGCCAGAGAAGTACGTT	55.0	45.0	57.8	57.1	2958-2998 bp	0.82	72.8
ndhF_p4	GGAAGAGATCCGAGTGAACG	TTTTGGCCAGAGAAGTACGT	55.0	45.0	57.8	57.1	2959-2999 bp	0.82	72.8
ndhF_p5	GGAAGAGATCCGAGTGAACG	CGCTTTTTGGCCAGAGAAGT	55.0	50.0	57.8	59.0	2963-2999 bp	0.78	70.2