Hotspot

rps15-ycf1

SSC · 120526-126060 · 5535 bp

Back to Rhodiola

Pi 0.0113

MarkerSeek score 61.00

Primer available yes

Label rps15-ycf1

Feature Metadata

label_name: rps15-ycf1
feature_type: igs
region: SSC
length_bp: 5535
variable_sites: 518
pi: 0.0113
unique_haplotype_count: 50
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps15-ycf1_p1	TCCCTTTGTGATTTGATAATGAGT	AGATGGTCTGGATTGGTACCA	33.3	47.6	56.3	58.2	175 bp	1.0	48.0
rps15-ycf1_p2	TCCCTTTGTGATTTGATAATGAGT	GATGGTCTGGATTGGTACCA	33.3	50.0	56.3	56.6	174 bp	1.0	48.0
rps15-ycf1_p3	TCCCTTTGTGATTTGATAATGAGTT	AGATGGTCTGGATTGGTACCA	32.0	47.6	57.0	58.2	175 bp	1.0	48.0
rps15-ycf1_p4	TTCCCTTTGTGATTTGATAATGAGT	AGATGGTCTGGATTGGTACCA	32.0	47.6	57.0	58.2	176 bp	1.0	48.0
rps15-ycf1_p5	CCCTTTGTGATTTGATAATGAGT	AGATGGTCTGGATTGGTACCA	34.8	47.6	54.9	58.2	174 bp	1.0	48.0