Hotspot

accD-psaI

LSC · 57278-57824 · 547 bp

Back to Rhodiola

Pi 0.00612

MarkerSeek score 66.60

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 547
variable_sites: 39
pi: 0.00612
unique_haplotype_count: 34
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GACCCAATCGTACCACGTAA	ATTGCCATTGCCGGAAAAAC	50.0	45.0	57.3	58.2	712-743 bp	1.0	82.4
accD-psaI_p2	GACCCAATCGTACCACGTAA	CATTGCCATTGCCGGAAAAA	50.0	45.0	57.3	58.2	713-744 bp	1.0	82.4
accD-psaI_p3	GACCCAATCGTACCACGTAA	GCCATTGCCGGAAAAACTAG	50.0	50.0	57.3	57.7	709-740 bp	1.0	81.7
accD-psaI_p4	GACCCAATCGTACCACGTAA	TGCCATTGCCGGAAAAACTA	50.0	45.0	57.3	58.4	710-741 bp	1.0	80.9
accD-psaI_p5	GACCCAATCGTACCACGTAA	AAGAAACCATTGCCATTGCC	50.0	45.0	57.3	57.5	720-751 bp	1.0	80.2