Hotspot

trnE-UUC-trnT-GGU

LSC · 30825-31666 · 842 bp

Back to Rhodiola

Pi 0.0128

MarkerSeek score 69.40

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 842
variable_sites: 72
pi: 0.0128
unique_haplotype_count: 41
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	CATAGTACCCTACCCCCAGG	GAACCGATGACTTACGCCTT	60.0	50.0	58.0	58.0	952-1023 bp	0.98	88.7
trnE-UUC-trnT-GGU_p2	CATAGTACCCTACCCCCAGG	CCATGGCGTTACTCTACCAC	60.0	55.0	58.0	58.1	931-1002 bp	0.98	88.4
trnE-UUC-trnT-GGU_p3	CATAGTACCCTACCCCCAGG	TGGCGTTACTCTACCACTGA	60.0	50.0	58.0	58.1	928-999 bp	0.98	88.2
trnE-UUC-trnT-GGU_p4	CATAGTACCCTACCCCCAGG	CCGATGACTTACGCCTTACC	60.0	55.0	58.0	58.2	949-1020 bp	0.98	87.6
trnE-UUC-trnT-GGU_p5	CATAGTACCCTACCCCCAGG	AAACACGAAAGCCCCCTATC	60.0	50.0	58.0	57.9	978-1049 bp	0.58	69.7