Hotspot

rpoB-trnC-GCA

LSC · 26102-27102 · 1001 bp

Back to Rhodiola

Pi 0.021

MarkerSeek score 75.60

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1001
variable_sites: 140
pi: 0.021
unique_haplotype_count: 46
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	AGTTCTTCCGTCAAGCCATG	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1110-1200 bp	1.0	89.9
rpoB-trnC-GCA_p2	GTTCTTCCGTCAAGCCATGA	CCGGATTTGAACTGGGGAAA	50.0	50.0	58.2	58.1	1109-1199 bp	1.0	89.8
rpoB-trnC-GCA_p3	AGTTCTTCCGTCAAGCCATG	AGTCTTGTGTTGATCAGGCG	50.0	50.0	58.2	58.2	1134-1224 bp	1.0	88.9
rpoB-trnC-GCA_p4	AGTTCTTCCGTCAAGCCATG	GTCTTGTGTTGATCAGGCGA	50.0	50.0	58.2	58.2	1133-1223 bp	1.0	88.9
rpoB-trnC-GCA_p5	AGTTCTTCCGTCAAGCCATG	TCTTGTGTTGATCAGGCGAC	50.0	50.0	58.2	58.2	1132-1222 bp	1.0	88.9