Hotspot

trnS-GCU-trnG-GCC

LSC · 7899-8531 · 633 bp

Back to Rhodiola

Pi 0.02
MarkerSeek score 70.30
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
633
variable_sites
91
pi
0.02
unique_haplotype_count
45
primer_available
yes
alignment_reliability
0.99
markerseek_score
70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 CAATCCGACGCTTTAGTCCA TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 600-703 bp 1.0 59.9
trnS-GCU-trnG-GCC_p2 AATCCGACGCTTTAGTCCAC TACCACTAAACTATACCCGC 50.0 45.0 58.0 54.0 599-702 bp 1.0 59.9
trnS-GCU-trnG-GCC_p3 GCTCGTACAACGGATTAGCA TACCACTAAACTATACCCGC 50.0 45.0 58.1 54.0 618-721 bp 1.0 59.2
trnS-GCU-trnG-GCC_p4 CAATCCGACGCTTTAGTCCA ACGAATCTCACTTTTACCACT 50.0 38.1 58.0 54.9 614-717 bp 1.0 59.1
trnS-GCU-trnG-GCC_p5 AATCCGACGCTTTAGTCCAC ACGAATCTCACTTTTACCACT 50.0 38.1 58.0 54.9 613-716 bp 1.0 59.1