Hotspot

matK-trnK-UUU

LSC · 3410-4111 · 702 bp

Back to Rhodiola

Pi 0.0121

MarkerSeek score 72.10

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 702
variable_sites: 68
pi: 0.0121
unique_haplotype_count: 40
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	CGGACCTATGATCATGAGCA	AACGGTAGAGTACTCGGCTT	50.0	50.0	56.9	58.2	823-853 bp	0.98	79.3
matK-trnK-UUU_p2	CGGACCTATGATCATGAGCA	ACGGTAGAGTACTCGGCTTT	50.0	50.0	56.9	58.2	822-852 bp	0.98	79.3
matK-trnK-UUU_p3	ACGGACCTATGATCATGAGC	AACGGTAGAGTACTCGGCTT	50.0	50.0	56.9	58.2	824-854 bp	0.98	79.2
matK-trnK-UUU_p4	ACGGACCTATGATCATGAGC	ACGGTAGAGTACTCGGCTTT	50.0	50.0	56.9	58.2	823-853 bp	0.98	79.2
matK-trnK-UUU_p5	CGGACCTATGATCATGAGCA	TTTTGGCTTGGGGTTGCTAA	50.0	45.0	56.9	58.2	846-876 bp	0.98	79.0