Hotspot

rps3

LSC · 91457-92134 · 678 bp

Back to Rhizophora

Pi 0.00393

MarkerSeek score 61.30

Primer available yes

Label rps3

Feature Metadata

label_name: rps3
feature_type: gene
region: LSC
length_bp: 678
variable_sites: 4
pi: 0.00393
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3_p1	TGGCTAGAACGTAAAAATGACA	TCAAGGTGGAAAAATAAGTCCA	36.4	36.4	56.0	55.5	764-782 bp	1.0	40.4
rps3_p2	TGGCTAGAACGTAAAAATGACA	AGTCCACAGATACAACATTTCA	36.4	36.4	56.0	55.4	748-766 bp	1.0	40.4
rps3_p3	TGGCTAGAACGTAAAAATGACAAG	TCAAGGTGGAAAAATAAGTCCA	37.5	36.4	57.8	55.5	764-782 bp	1.0	40.4
rps3_p4	TGGCTAGAACGTAAAAATGACAA	TCAAGGTGGAAAAATAAGTCCA	34.8	36.4	56.7	55.5	764-782 bp	1.0	40.4
rps3_p5	TGGCTAGAACGTAAAAATGACA	AAGTCCACAGATACAACATTTCA	36.4	34.8	56.0	56.1	749-767 bp	1.0	40.4