Hotspot

rps19

LSC · 251-505 · 255 bp

Back to Rhizophora

Pi 0.0444

MarkerSeek score 38.80

Primer available yes

Label rps19

Feature Metadata

label_name: rps19
feature_type: gene
region: LSC
length_bp: 255
variable_sites: 3
pi: 0.0444
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.18
markerseek_score: 38.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19_p1	GGGCCCCAATTGGTAGAAAA	GCCCATAATCACTTCGCTCA	50.0	50.0	58.1	58.0	620 bp	0.333333	52.5
rps19_p2	GGGCCCCAATTGGTAGAAAA	GTCGTTCGCCCATAATCACT	50.0	50.0	58.1	58.1	627 bp	0.333333	52.4
rps19_p3	TTGATTCTTCGTCGCCGTAG	GCCCATAATCACTTCGCTCA	50.0	50.0	58.1	58.0	528 bp	0.333333	52.2
rps19_p4	TTGATTCTTCGTCGCCGTAG	GTCGTTCGCCCATAATCACT	50.0	50.0	58.1	58.1	535 bp	0.333333	52.1
rps19_p5	GGGCCCCAATTGGTAGAAAA	CACTTCGCTCATAACCCCAA	50.0	50.0	58.1	57.9	611 bp	0.333333	52.0