Hotspot

rpl32-trnL-TAG

SSC · 122469-124084 · 1616 bp

Back to Rhizophora

Pi 0.00735

MarkerSeek score 59.80

Primer available yes

Label rpl32-trnL-TAG

Feature Metadata

label_name: rpl32-trnL-TAG
feature_type: igs
region: SSC
length_bp: 1616
variable_sites: 15
pi: 0.00735
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-TAG_p1	GGATATTGGGCAGCCTTGAA	AGCGTGTCTACCGATTTCAC	50.0	50.0	57.9	58.0	1718-1750 bp	1.0	84.0
rpl32-trnL-TAG_p2	GGATATTGGGCAGCCTTGAA	CAGCGTGTCTACCGATTTCA	50.0	50.0	57.9	58.0	1719-1751 bp	1.0	84.0
rpl32-trnL-TAG_p3	GGATATTGGGCAGCCTTGAA	TCCTAAGAGCAGCGTGTCTA	50.0	50.0	57.9	57.9	1728-1760 bp	1.0	83.0
rpl32-trnL-TAG_p4	GGATATTGGGCAGCCTTGAA	AAAGATGTTATGCCGCCACT	50.0	45.0	57.9	57.9	1779-1811 bp	1.0	82.9
rpl32-trnL-TAG_p5	GGCAGCCTTGAAAGCTTTTT	AGCGTGTCTACCGATTTCAC	45.0	50.0	57.8	58.0	1710-1742 bp	1.0	82.6