Hotspot

ndhF-rpl32

SSC · 121267-122294 · 1028 bp

Back to Rhizophora

Pi 0.00206
MarkerSeek score 64.40
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1028
variable_sites
3
pi
0.00206
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.94
markerseek_score
64.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 CGGAAAAAGTAGGAGTCCCG TTCAAGGCTGCCCAATATCC 55.0 50.0 58.0 57.9 1151-1207 bp 1.0 83.6
ndhF-rpl32_p2 CGGAAAAAGTAGGAGTCCCG AAAAAGCTTTCAAGGCTGCC 55.0 45.0 58.0 57.8 1159-1215 bp 1.0 82.2
ndhF-rpl32_p3 GGAAAAAGTAGGAGTCCCGC TTCAAGGCTGCCCAATATCC 55.0 50.0 58.3 57.9 1150-1206 bp 1.0 81.5
ndhF-rpl32_p4 GAGTCCCGCTCCTATTACCA TTCAAGGCTGCCCAATATCC 55.0 50.0 58.3 57.9 1139-1195 bp 1.0 81.2
ndhF-rpl32_p5 CGGAAAAAGTAGGAGTCCCG GCTGCCCAATATCCCTTTCT 55.0 50.0 58.0 57.6 1145-1201 bp 1.0 81.2